› 5kvy

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF THE TWO TANDEM RRM DOMAINS OF PUF60 BOUND TO A PORTION OF AN ADML PRE-MRNA 3' SPLICE SITE ANALOG

Released:
DOI: 10.2210/pdb5kvy/pdb
PDB entry 5kvy coloured by chain, front view.
PDB entry 5kvy coloured by chain, side view.
PDB entry 5kvy coloured by chain, top view.
Source organisms:
Primary publication:
Unraveling the mechanism of recognition of the 3' splice site of the adenovirus major late promoter intron by the alternative splicing factor PUF60.
Hsiao HT, Crichlow GV, Murphy JW, Folta-Stogniew EJ, Lolis EJ, Braddock DT
PLoS One 15 e0242725 (2020)
PMID: 33253191

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118970 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Poly(U)-binding-splicing factor PUF60 Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHX1 (Residues: 118-316; Coverage: 36%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
DNA (30-MER) Chain: C
Molecule details ›
Chain: C
Length: 30 nucleotides
Theoretical weight: 8.86 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P31
Unit cell:
a: 62.438Å b: 62.438Å c: 83.332Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.232
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 citations in other articles

Decreased spliceosome fidelity and egl-8 intron retention inhibit mTORC1 signaling to promote longevity.
Huang et al. (2022)

3 mentions without citation

Structural and biochemical insights into small RNA 3' end trimming by Arabidopsis SDN1.
Chen et al. (2018)
2 more