› 5kw2

X-ray diffraction
2.76Å resolution

The extra-helical binding site of GPR40 and the structural basis for allosteric agonism and incretin stimulation

Released:
DOI: 10.2210/pdb5kw2/pdb
PDB entry 5kw2 coloured by chain, front view.
PDB entry 5kw2 coloured by chain, side view.
PDB entry 5kw2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for GPR40 allosteric agonism and incretin stimulation.
Ho JD, Chau B, Rodgers L, Lu F, Wilbur KL, Otto KA, Chen Y, Song M, Riley JP, Yang HC, Reynolds NA, Kahl SD, Lewis AP, Groshong C, Madsen RE, Conners K, Lineswala JP, Gheyi T, Saflor MD, Lee MR, Benach J, Baker KA, Montrose-Rafizadeh C, Genin MJ, Miller AR, Hamdouchi C
Nat Commun 9 1645 (2018)
PMID: 29695780

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Free fatty acid receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 53.1 KDa
Source organisms: Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: O14842 (Residues: 1-211, 214-300; Coverage: 99%)
  • Canonical: P00720 (Residues: 2-161; Coverage: 98%)
Gene names: E, FFAR1, GPR40
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 6XQ 1 x 6XQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 147.654Å b: 55.95Å c: 80.313Å
α: 90° β: 93.77° γ: 90°
R-values:
R R work R free
0.233 0.23 0.273
Expression system: Baculovirus expression vector pFastBac1-HM

Quick links

Citations

5 review citations

Targeting lipid GPCRs to treat type 2 diabetes mellitus - progress and challenges.
Ghislain et al. (2021)
4 more

14 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
13 more