› 5kwd

X-ray diffraction
2.75Å resolution

Computationally Designed Symmetric Homotetramer

Released:
DOI: 10.2210/pdb5kwd/pdb
PDB entry 5kwd coloured by chain, front view.
PDB entry 5kwd coloured by chain, side view.
PDB entry 5kwd coloured by chain, top view.
Source organism: Metallosphaera yellowstonensis MK1
Primary publication:
Computational design of self-assembling cyclic protein homo-oligomers.
Fallas JA, Ueda G, Sheffler W, Nguyen V, McNamara DE, Sankaran B, Pereira JH, Parmeggiani F, Brunette TJ, Cascio D, Yeates TR, Zwart P, Baker D
Nat Chem 9 353-360 (2017)
PMID: 28338692

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165914 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ankyrin repeat-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 160 amino acids
Theoretical weight: 17.36 KDa
Source organism: Metallosphaera yellowstonensis MK1
Expression system: Escherichia coli BL21
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 77.77Å b: 89.47Å c: 99.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.229 0.281
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
9 more

1 mention without citation

Computational design of self-assembling cyclic protein homo-oligomers.
Fallas et al. (2017)