5ky4

X-ray diffraction
1.47Å resolution

mouse POFUT1 in complex with mouse Notch1 EGF26 and GDP

Released:
Source organism: Mus musculus

Function and Biology Details

Reaction catalysed:
GDP-beta-L-fucose + [protein]-(L-serine/L-threonine) = GDP + [protein]-3-O-(alpha-L-fucosyl)-(L-serine/L-threonine)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-169618 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GDP-fucose protein O-fucosyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.46 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q91ZW2 (Residues: 33-384; Coverage: 97%)
Gene name: Pofut1
Sequence domains: GDP-fucose protein O-fucosyltransferase
Structure domains:
Neurogenic locus notch homolog protein 1 Chain: B
Molecule details ›
Chain: B
Length: 40 amino acids
Theoretical weight: 4.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01705 (Residues: 983-1022; Coverage: 2%)
Gene names: Motch, Notch1
Sequence domains: Human growth factor-like EGF

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 51.92Å b: 66.372Å c: 110.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.168
Expression systems:
  • Homo sapiens
  • Escherichia coli