› 5kyt

X-ray diffraction
2Å resolution

Structure of Photinus pyralis Luciferase red light emitting variant

Released:
DOI: 10.2210/pdb5kyt/pdb
PDB entry 5kyt coloured by chain, front view.
PDB entry 5kyt coloured by chain, side view.
PDB entry 5kyt coloured by chain, top view.
Source organism: Photinus pyralis
Primary publication:
Cloning of the Orange Light-Producing Luciferase from Photinus scintillans-A New Proposal on how Bioluminescence Color is Determined.
Branchini BR, Southworth TL, Fontaine DM, Murtiashaw MH, McGurk A, Talukder MH, Qureshi R, Yetil D, Sundlov JA, Gulick AM
Photochem Photobiol 93 479-485 (2017)
PMID: 27861940

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 550 amino acids
Theoretical weight: 60.83 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08659 (Residues: 1-550; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SLU 2 x SLU
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P3121
Unit cell:
a: 92.587Å b: 92.587Å c: 294.465Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Advances in bioluminescence imaging: new probes from old recipes.
Yao et al. (2018)
1 more

3 mentions without citation

Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging.
Zambito et al. (2021)
2 more