› 5kyv

X-ray diffraction
2.5Å resolution

Structure of Photinus pyralis Luciferase green shifted light emitting variant

Released:
DOI: 10.2210/pdb5kyv/pdb
PDB entry 5kyv coloured by chain, front view.
PDB entry 5kyv coloured by chain, side view.
PDB entry 5kyv coloured by chain, top view.
Source organism: Photinus pyralis
Entry author: Gulick AM

Function and Biology Details

Reaction catalysed: 
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 550 amino acids
Theoretical weight: 60.79 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08659 (Residues: 1-550; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SLU 2 x SLU
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P3121
Unit cell:
a: 93.078Å b: 93.078Å c: 293.645Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.194 0.248
Expression system: Escherichia coli BL21(DE3)

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