› 5l08

Electron Microscopy
4.6Å resolution

Cryo-EM structure of Casp-8 tDED filament

Released:
DOI: 10.2210/pdb5l08/pdb
PDB entry 5l08 coloured by chain, front view.
PDB entry 5l08 coloured by chain, side view.
PDB entry 5l08 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM Structure of Caspase-8 Tandem DED Filament Reveals Assembly and Regulation Mechanisms of the Death-Inducing Signaling Complex.
Fu TM, Li Y, Lu A, Li Z, Vajjhala PR, Cruz AC, Srivastava DB, DiMaio F, Penczek PA, Siegel RM, Stacey KJ, Egelman EH, Wu H
Mol Cell 64 236-250 (2016)
PMID: 27746017
Related structures: EMD-8300

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase-8 Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 184 amino acids
Theoretical weight: 22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14790 (Residues: 1-184; Coverage: 38%)
Gene names: CASP8, MCH5
Sequence domains: Death effector domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.6Å
Relevant EMDB volumes: EMD-8300
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018.
Galluzzi et al. (2018)
39 more

7 mentions without citation

Higher-order assemblies in immune signaling: supramolecular complexes and phase separation.
Xia et al. (2021)
6 more