› 5l26

X-ray diffraction
3.4Å resolution

Structure of CNTnw in an inward-facing substrate-bound state

Released:
DOI: 10.2210/pdb5l26/pdb
PDB entry 5l26 coloured by chain, front view.
PDB entry 5l26 coloured by chain, side view.
PDB entry 5l26 coloured by chain, top view.
Source organism: Neisseria wadsworthii 9715
Primary publication:
Visualizing multistep elevator-like transitions of a nucleoside transporter.
Hirschi M, Johnson ZL, Lee SY
Nature 545 66-70 (2017)
PMID: 28424521

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside permease Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 431 amino acids
Theoretical weight: 44.67 KDa
Source organism: Neisseria wadsworthii 9715
Expression system: Escherichia coli
UniProt:
  • Canonical: G4CRQ5 (Residues: 1-425; Coverage: 100%)
Gene names: HMPREF9370_1765, nupC
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand 6ZL 9 x 6ZL
Ligand URI 3 x URI
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P61
Unit cell:
a: 115.22Å b: 115.22Å c: 264.028Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.242 0.266
Expression system: Escherichia coli

Quick links

Citations

3 review citations

General principles of secondary active transporter function.
Beckstein et al. (2022)
2 more

6 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
5 more