› 5l27

X-ray diffraction
4.1Å resolution

Structure of CNTnw N149L in the intermediate 1 state

Released:
DOI: 10.2210/pdb5l27/pdb
PDB entry 5l27 coloured by chain, front view.
PDB entry 5l27 coloured by chain, side view.
PDB entry 5l27 coloured by chain, top view.
Source organism: Neisseria wadsworthii 9715
Primary publication:
Visualizing multistep elevator-like transitions of a nucleoside transporter.
Hirschi M, Johnson ZL, Lee SY
Nature 545 66-70 (2017)
PMID: 28424521

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside permease Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 431 amino acids
Theoretical weight: 44.67 KDa
Source organism: Neisseria wadsworthii 9715
Expression system: Escherichia coli
UniProt:
  • Canonical: G4CRQ5 (Residues: 1-425; Coverage: 100%)
Gene names: HMPREF9370_1765, nupC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 6ZL 1 x 6ZL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 116.1Å b: 116.1Å c: 272.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.272 0.269 0.309
Expression system: Escherichia coli

Quick links

Citations

3 review citations

General principles of secondary active transporter function.
Beckstein et al. (2022)
2 more

5 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
4 more