› 5l32

X-ray diffraction
2.1Å resolution

Crystal structure of the Zn-RIDC1 complex bearing six interfacial disulfide bonds

Released:
DOI: 10.2210/pdb5l32/pdb
PDB entry 5l32 coloured by chain, front view.
PDB entry 5l32 coloured by chain, side view.
PDB entry 5l32 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
De Novo Design of an Allosteric Metalloprotein Assembly with Strained Disulfide Bonds.
Churchfield LA, Medina-Morales A, Brodin JD, Perez A, Tezcan FA
J Am Chem Soc 138 13163-13166 (2016)
PMID: 27649076

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

3 bound ligands:
Ligand HEC 4 x HEC
Ligand P6G 1 x P6G
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21
Unit cell:
a: 48.06Å b: 62.533Å c: 72.437Å
α: 90° β: 99.01° γ: 90°
R-values:
R R work R free
0.216 0.214 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Allosteric disulfides: Sophisticated molecular structures enabling flexible protein regulation.
Chiu et al. (2019)
3 more

2 mentions without citation

De Novo Design of an Allosteric Metalloprotein Assembly with Strained Disulfide Bonds.
Churchfield et al. (2016)
1 more