› 5l4o

X-ray diffraction
2.8Å resolution

Structure of an E.coli initiator tRNAfMet A1-U72 variant

Released:
DOI: 10.2210/pdb5l4o/pdb
PDB entry 5l4o coloured by chain, front view.
PDB entry 5l4o coloured by chain, side view.
PDB entry 5l4o coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of an E. coli tRNAfMet A1-U72 variant shows an unusual conformation of the A1-U72 base pair.
Monestier A, Aleksandrov A, Coureux PD, Panvert M, Mechulam Y, Schmitt E
RNA 23 673-682 (2017)
PMID: 28143889

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195823 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
tRNA (76-MER) Chain: A
Molecule details ›
Chain: A
Length: 77 nucleotides
Theoretical weight: 24.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Sequence domains: tRNA

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
5 modified residues:
Ligand 4SU 1 x 4SU
Ligand H2U 1 x H2U
Ligand OMC 1 x OMC
Ligand 5MU 1 x 5MU
Ligand PSU 1 x PSU

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 35.15Å b: 142.62Å c: 202.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.226
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Start Codon Recognition in Eukaryotic and Archaeal Translation Initiation: A Common Structural Core.
Schmitt et al. (2019)
4 other articles

5 mentions without citation

The structure of an E. coli tRNAfMet A1-U72 variant shows an unusual conformation of the A1-U72 base pair.
Monestier et al. (2017)
4 more