› 5lba

X-ray diffraction
2.5Å resolution

Crystal structure of human RECQL5 helicase in complex with DSPL fragment(1-cyclohexyl-3-(oxolan-2-ylmethyl)urea, SGC - Diamond XChem I04-1 fragment screening.

Released:
DOI: 10.2210/pdb5lba/pdb
PDB entry 5lba coloured by chain, front view.
PDB entry 5lba coloured by chain, side view.
PDB entry 5lba coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Newman JA, Aitkenhead H, Talon R, Savitsky P, Krojer T, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Gileadi O, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase Q5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 445 amino acids
Theoretical weight: 49.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O94762 (Residues: 11-453; Coverage: 45%)
Gene names: RECQ5, RECQL5
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

5 bound ligands:
Ligand MG 4 x MG
Ligand ZN 4 x ZN
Ligand ADP 4 x ADP
Ligand DMS 3 x DMS
Ligand 6SV 1 x 6SV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 63.926Å b: 85.261Å c: 105.915Å
α: 109.9° β: 90.02° γ: 97.01°
R-values:
R R work R free
0.24 0.237 0.303
Expression system: Escherichia coli BL21

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