› 5ld8

X-ray diffraction
2.13Å resolution

Function and Biology Details

Reaction catalysed: 
An ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO(2) + a holo-[acyl-carrier protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 435 amino acids
Theoretical weight: 45.4 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WQD9 (Residues: 2-416; Coverage: 100%)
Gene names: MTCY427.26, Rv2245, kasA
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

3 bound ligands:
Ligand NA 2 x NA
Ligand 6U5 2 x 6U5
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P31
Unit cell:
a: 77.338Å b: 77.338Å c: 147.675Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.161 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Mycobacterial cell wall biosynthesis: a multifaceted antibiotic target.
Abrahams et al. (2018)
15 more

3 mentions without citation

Antibiotics and resistance: the two-sided coin of the mycobacterial cell wall.
Batt et al. (2020)
2 more