› 5lg3

X-ray diffraction
3.57Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with chlorpromazine

Released:
DOI: 10.2210/pdb5lg3/pdb
PDB entry 5lg3 coloured by chain, front view.
PDB entry 5lg3 coloured by chain, side view.
PDB entry 5lg3 coloured by chain, top view.
Source organism: Dickeya dadantii 3937
Primary publication:
Allosteric binding site in a Cys-loop receptor ligand-binding domain unveiled in the crystal structure of ELIC in complex with chlorpromazine.
Nys M, Wijckmans E, Farinha A, Yoluk Ö, Andersson M, Brams M, Spurny R, Peigneur S, Tytgat J, Lindahl E, Ulens C
Proc Natl Acad Sci U S A 113 E6696-E6703 (2016)
PMID: 27791038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 307 amino acids
Theoretical weight: 35.38 KDa
Source organism: Dickeya dadantii 3937
Expression system: Escherichia coli
UniProt:
  • Canonical: E0SJQ4 (Residues: 32-338; Coverage: 95%)
Gene name: Dda3937_00520
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand Z80 10 x Z80
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 105.2Å b: 266.65Å c: 111.04Å
α: 90° β: 107.71° γ: 90°
R-values:
R R work R free
0.222 0.22 0.253
Expression system: Escherichia coli

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Citations

1 review citation

Nicotinic acetylcholine receptors at the single-channel level.
Bouzat et al. (2018)
10 other articles

9 mentions without citation

Laminin α5 guides tissue patterning and organogenesis.
Spenlé et al. (2013)
8 more