› 5lgs

X-ray diffraction
2.1Å resolution

Crystal structure of mouse CARM1 in complex with ligand P2C3u

Released:
DOI: 10.2210/pdb5lgs/pdb
Model geometry
Fit model/data
PDB entry 5lgs coloured by chain, front view.
PDB entry 5lgs coloured by chain, side view.
PDB entry 5lgs coloured by chain, top view.
Source organisms:
Primary publication:
Transition state mimics are valuable mechanistic probes for structural studies with the arginine methyltransferase CARM1.
van Haren MJ, Marechal N, Troffer-Charlier N, Cianciulli A, Sbardella G, Cavarelli J, Martin NI
Proc Natl Acad Sci U S A 114 3625-3630 (2017)
PMID: 28330993

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146005 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-arginine methyltransferase CARM1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 361 amino acids
Theoretical weight: 40.85 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9WVG6 (Residues: 130-487; Coverage: 59%)
Gene names: Carm1, Prmt4
Sequence domains:
Structure domains:
Polyadenylate-binding protein 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 9 amino acids
Theoretical weight: 940 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P11940 (Residues: 456-464; Coverage: 1%)
Gene names: PAB1, PABP, PABP1, PABPC1, PABPC2

Ligands and Environments

6 bound ligands:
Ligand SO4 1 x SO4
Ligand DXE 3 x DXE
Ligand PEG 1 x PEG
Ligand PG4 1 x PG4
Ligand EDO 3 x EDO
Ligand QVR 4 x QVR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 74.986Å b: 98.596Å c: 208.172Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.215
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

3 review citations

Protein Arginine Methyltransferases as Therapeutic Targets in Hematological Malignancies.
Sauter et al. (2022)
2 more

3 mentions without citation

Protein arginine methyltransferases: insights into the enzyme structure and mechanism at the atomic level.
Tewary et al. (2019)
2 more