› 5lid

X-ray diffraction
4.5Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromopromazine

Released:
DOI: 10.2210/pdb5lid/pdb
PDB entry 5lid coloured by chain, front view.
PDB entry 5lid coloured by chain, side view.
PDB entry 5lid coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
Allosteric binding site in a Cys-loop receptor ligand-binding domain unveiled in the crystal structure of ELIC in complex with chlorpromazine.
Nys M, Wijckmans E, Farinha A, Yoluk Ö, Andersson M, Brams M, Spurny R, Peigneur S, Tytgat J, Lindahl E, Ulens C
Proc Natl Acad Sci U S A 113 E6696-E6703 (2016)
PMID: 27791038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cys-loop ligand-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 307 amino acids
Theoretical weight: 35.38 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C7B7 (Residues: 11-163, 164-316; Coverage: 95%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

1 bound ligand:
Ligand 6XY 20 x 6XY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 105.72Å b: 267.91Å c: 111.33Å
α: 90° β: 106.7° γ: 90°
R-values:
R R work R free
0.215 0.212 0.257
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Nicotinic acetylcholine receptors at the single-channel level.
Bouzat et al. (2018)
1 more

2 mentions without citation

Allosteric binding site in a Cys-loop receptor ligand-binding domain unveiled in the crystal structure of ELIC in complex with chlorpromazine.
Nys et al. (2016)
1 more