› 5ljw

X-ray diffraction
1.8Å resolution

MamK non-polymerising A278D mutant bound to AMPPNP

Released:
DOI: 10.2210/pdb5ljw/pdb
PDB entry 5ljw coloured by chain, front view.
PDB entry 5ljw coloured by chain, side view.
PDB entry 5ljw coloured by chain, top view.
Source organism: Magnetospirillum magneticum AMB-1
Primary publication:
X-ray and cryo-EM structures of monomeric and filamentous actin-like protein MamK reveal changes associated with polymerization.
Löwe J, He S, Scheres SH, Savva CG
Proc Natl Acad Sci U S A 113 13396-13401 (2016)
PMID: 27821762

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-like protein MamK Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 37.69 KDa
Source organism: Magnetospirillum magneticum AMB-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2W8Q6 (Residues: 1-347; Coverage: 100%)
Gene names: amb0965, mamK
Sequence domains: MreB/Mbl protein
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 72.572Å b: 71.803Å c: 79.761Å
α: 90° β: 98.41° γ: 90°
R-values:
R R work R free
0.18 0.178 0.206
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Prokaryotic cytoskeletons: protein filaments organizing small cells.
Wagstaff et al. (2018)
3 more

1 mention without citation

X-ray and cryo-EM structures of monomeric and filamentous actin-like protein MamK reveal changes associated with polymerization.
Löwe et al. (2016)