› 5lly

X-ray diffraction
2.4Å resolution

Photosensory Module of Bacteriophytochrome linked Diguanylyl Cyclase from Idiomarina species A28L

Released:
DOI: 10.2210/pdb5lly/pdb
Model geometry
Fit model/data
PDB entry 5lly coloured by chain, front view.
PDB entry 5lly coloured by chain, side view.
PDB entry 5lly coloured by chain, top view.
Source organism: Idiomarina sp. A28L
Primary publication:
Long-range allosteric signaling in red light-regulated diguanylyl cyclases.
Gourinchas G, Etzl S, Göbl C, Vide U, Madl T, Winkler A
OpenAccess logo Sci Adv 3 e1602498 (2017)
PMID: 28275738

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 529 amino acids
Theoretical weight: 60.14 KDa
Source organism: Idiomarina sp. A28L
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F7RW09 (Residues: 3-527; Coverage: 77%)
Gene name: A28LD_0430
Sequence domains:
Structure domains: Beta-Lactamase

Ligands and Environments

3 bound ligands:
Ligand LBV 4 x LBV
Ligand CL 8 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 83.5Å b: 129Å c: 122.01Å
α: 90° β: 96.34° γ: 90°
R-values:
R R work R free
0.18 0.177 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Signal transduction in photoreceptor histidine kinases.
Möglich A. (2019)
9 more

2 mentions without citation

Phytochromes in Agrobacterium fabrum.
Lamparter et al. (2021)
1 more