› 5lm2

X-ray diffraction
2.54Å resolution

Crystal Structure of HD-PTP phosphatase

Released:
DOI: 10.2210/pdb5lm2/pdb
PDB entry 5lm2 coloured by chain, front view.
PDB entry 5lm2 coloured by chain, side view.
PDB entry 5lm2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Selective Interaction between the ESCRT Regulator HD-PTP and UBAP1.
Gahloth D, Levy C, Heaven G, Stefani F, Wunderley L, Mould P, Cliff MJ, Bella J, Fielding AJ, Woodman P, Tabernero L
Structure 24 2115-2126 (2016)
PMID: 27839950

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 23 Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 40.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H3S7 (Residues: 362-713; Coverage: 22%)
Gene names: KIAA1471, PTPN23
Sequence domains: ALIX V-shaped domain binding to HIV

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 53.49Å b: 47.65Å c: 172.71Å
α: 90° β: 96.01° γ: 90°
R-values:
R R work R free
0.219 0.215 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The Roles of Pseudophosphatases in Disease.
Mattei et al. (2021)
2 more

2 mentions without citation

The open architecture of HD-PTP phosphatase provides new insights into the mechanism of regulation of ESCRT function.
Gahloth et al. (2017)
1 more