› 5lne

X-ray diffraction
2.2Å resolution

E. coli F9 pilus adhesin FmlH bound to the Thomsen-Friedenreich (TF) antigen

Released:
DOI: 10.2210/pdb5lne/pdb
PDB entry 5lne coloured by chain, front view.
PDB entry 5lne coloured by chain, side view.
PDB entry 5lne coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Inflammation-Induced Adhesin-Receptor Interaction Provides a Fitness Advantage to Uropathogenic E. coli during Chronic Infection.
Conover MS, Ruer S, Taganna J, Kalas V, De Greve H, Pinkner JS, Dodson KW, Remaut H, Hultgren SJ
Cell Host Microbe 20 482-492 (2016)
PMID: 27667696

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
FimH mannose-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 17.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0H2V7F6 (Residues: 25-182; Coverage: 52%)
Gene names: c1931, ydeQ
Sequence domains: FimH, mannose binding
Structure domains: Fimbrial-type adhesion domain

Ligands and Environments

Carbohydrate polymer : NEW Components: A2G, GAL
Carbohydrate polymer
1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21212
Unit cell:
a: 118.82Å b: 51.46Å c: 53.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Classical chaperone-usher (CU) adhesive fimbriome: uropathogenic Escherichia coli (UPEC) and urinary tract infections (UTIs).
Behzadi P. (2020)
13 more