› 5lnk

Electron Microscopy
3.9Å resolution

Entire ovine respiratory complex I

Released:
DOI: 10.2210/pdb5lnk/pdb
PDB entry 5lnk coloured by chain, front view.
PDB entry 5lnk coloured by chain, side view.
PDB entry 5lnk coloured by chain, top view.
Source organism: Ovis aries
Primary publication:
Atomic structure of the entire mammalian mitochondrial complex I.
Fiedorczuk K, Letts JA, Degliesposti G, Kaszuba K, Skehel M, Sazanov LA
Nature 538 406-410 (2016)
PMID: 27595392
Related structures: EMD-4093

Function and Biology Details

Reaction catalysed: 
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 45-mer (preferred)
PDBe Complex ID:
PDB-CPX-131275 (preferred)
Entry contents:
44 distinct polypeptide molecules
Macromolecules (44 distinct):
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: 1
Molecule details ›
Chain: 1
Length: 445 amino acids
Theoretical weight: 48.68 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PUX0 (Residues: 20-464; Coverage: 96%)
Gene name: NDUFV1
Sequence domains:
Structure domains:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: 2
Molecule details ›
Chain: 2
Length: 217 amino acids
Theoretical weight: 23.84 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NRY1 (Residues: 30-246; Coverage: 88%)
Gene name: NDUFV2
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
Mitochondrial complex I, 75 kDa subunit Chain: 3
Molecule details ›
Chain: 3
Length: 704 amino acids
Theoretical weight: 77.03 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Chain: 4
Molecule details ›
Chain: 4
Length: 412 amino acids
Theoretical weight: 47.12 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PJ73 (Residues: 1-412; Coverage: 100%)
Gene name: NDUFS2
Sequence domains: Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Structure domains: Cytochrome-c3 Hydrogenase, chain B
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial Chain: 5
Molecule details ›
Chain: 5
Length: 228 amino acids
Theoretical weight: 26.44 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PB27 (Residues: 39-266; Coverage: 86%)
Gene name: NDUFS3
Sequence domains: Respiratory-chain NADH dehydrogenase, 30 Kd subunit
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Chain: 6
Molecule details ›
Chain: 6
Length: 179 amino acids
Theoretical weight: 20.1 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P42026 (Residues: 38-216; Coverage: 83%)
Gene name: NDUFS7
Sequence domains: NADH ubiquinone oxidoreductase, 20 Kd subunit
Structure domains: Rossmann fold
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Chain: 9
Molecule details ›
Chain: 9
Length: 176 amino acids
Theoretical weight: 20.22 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P42028 (Residues: 37-212; Coverage: 83%)
Gene name: NDUFS8
Sequence domains: 4Fe-4S dicluster domain
NADH-ubiquinone oxidoreductase chain 1 Chain: H
Molecule details ›
Chain: H
Length: 318 amino acids
Theoretical weight: 35.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78747 (Residues: 1-318; Coverage: 100%)
Gene names: MT-ND1, MTND1, NADH1, ND1
Sequence domains: NADH dehydrogenase
NADH-ubiquinone oxidoreductase chain 2 Chain: N
Molecule details ›
Chain: N
Length: 347 amino acids
Theoretical weight: 39.15 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78748 (Residues: 1-347; Coverage: 100%)
Gene names: MT-ND2, MTND2, NADH2, ND2
Sequence domains:
NADH-ubiquinone oxidoreductase chain 3 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.11 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78753 (Residues: 1-115; Coverage: 100%)
Gene names: MT-ND3, MTND3, NADH3, ND3
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Structure domains: NADH:ubiquinone/plastoquinone oxidoreductase, chain 3
NADH-ubiquinone oxidoreductase chain 4 Chain: M
Molecule details ›
Chain: M
Length: 459 amino acids
Theoretical weight: 52.06 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78755 (Residues: 1-459; Coverage: 100%)
Gene names: MT-ND4, MTND4, NADH4, ND4
Sequence domains:
NADH-ubiquinone oxidoreductase chain 4L Chain: K
Molecule details ›
Chain: K
Length: 98 amino acids
Theoretical weight: 10.84 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78754 (Residues: 1-98; Coverage: 100%)
Gene names: MT-ND4L, MTND4L, NADH4L, ND4L
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
Structure domains: Helix Hairpins
NADH-ubiquinone oxidoreductase chain 5 Chain: L
Molecule details ›
Chain: L
Length: 599 amino acids
Theoretical weight: 67.55 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78756 (Residues: 1-599; Coverage: 99%)
Gene names: MT-ND5, MTND5, NADH5, ND5
Sequence domains:
NADH-ubiquinone oxidoreductase chain 6 Chain: J
Molecule details ›
Chain: J
Length: 175 amino acids
Theoretical weight: 19.13 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: O78757 (Residues: 1-175; Coverage: 100%)
Gene names: MT-ND6, MTND6, NADH6, ND6
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial Chain: a
Molecule details ›
Chain: a
Length: 75 amino acids
Theoretical weight: 8.42 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P25712 (Residues: 35-109; Coverage: 69%)
Gene name: NDUFV3
Sequence domains: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Chain: b
Molecule details ›
Chain: b
Length: 96 amino acids
Theoretical weight: 10.65 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: P23934 (Residues: 29-124; Coverage: 77%)
Gene name: NDUFS6
Sequence domains: Zinc-finger domain
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: c
Molecule details ›
Chain: c
Length: 133 amino acids
Theoretical weight: 15.38 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PE07 (Residues: 38-170; Coverage: 78%)
Gene name: NDUFS4
Sequence domains: NADH dehydrogenase ubiquinone Fe-S protein 4
Mitochondrial complex I, 39 kDa subunit Chain: d
Molecule details ›
Chain: d
Length: 338 amino acids
Theoretical weight: 38.66 KDa
Source organism: Ovis aries
Mitochondrial complex I, B8 subunit Chain: e
Molecule details ›
Chain: e
Length: 98 amino acids
Theoretical weight: 10.97 KDa
Source organism: Ovis aries
Structure domains: Glutaredoxin
Mitochondrial complex I, B13 subunit Chain: f
Molecule details ›
Chain: f
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Chain: g
Molecule details ›
Chain: g
Length: 127 amino acids
Theoretical weight: 14.92 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QC06 (Residues: 14-140; Coverage: 91%)
Gene name: NDUFA6
Sequence domains: Complex1_LYR-like
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 Chain: h
Molecule details ›
Chain: h
Length: 112 amino acids
Theoretical weight: 12.4 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P0I2 (Residues: 3-114; Coverage: 98%)
Gene name: NDUFA7
Sequence domains: NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a)
Mitochondrial complex I, B17.2 subunit Chain: i
Molecule details ›
Chain: i
Length: 145 amino acids
Theoretical weight: 17.12 KDa
Source organism: Ovis aries
Acyl carrier protein Chains: X, j
Molecule details ›
Chains: X, j
Length: 88 amino acids
Theoretical weight: 10.12 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5NQT7 (Residues: 70-157; Coverage: 56%)
Sequence domains: Phosphopantetheine attachment site
Mitochondrial complex I, 42 kDa subunit Chain: k
Molecule details ›
Chain: k
Length: 320 amino acids
Theoretical weight: 36.8 KDa
Source organism: Ovis aries
Mitochondrial complex I, 15 kDa subunit Chain: l
Molecule details ›
Chain: l
Length: 105 amino acids
Theoretical weight: 12.47 KDa
Source organism: Ovis aries
Mitochondrial complex I, B9 subunit Chain: m
Molecule details ›
Chain: m
Length: 83 amino acids
Theoretical weight: 9.19 KDa
Source organism: Ovis aries
Mitochondrial complex I, B12 subunit Chain: n
Molecule details ›
Chain: n
Length: 97 amino acids
Theoretical weight: 11.02 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: o
Molecule details ›
Chain: o
Length: 120 amino acids
Theoretical weight: 14.23 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5P9Q8 (Residues: 3-122; Coverage: 98%)
Gene name: NDUFC2
Sequence domains: NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2)
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Chain: p
Molecule details ›
Chain: p
Length: 128 amino acids
Theoretical weight: 14.98 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QF73 (Residues: 3-130; Coverage: 99%)
Gene name: NDUFB4
Sequence domains: NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4)
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Chain: q
Molecule details ›
Chain: q
Length: 143 amino acids
Theoretical weight: 16.64 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: Q95KV7 (Residues: 2-144; Coverage: 99%)
Gene names: GRIM19, NDUFA13
Sequence domains: GRIM-19 protein
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Chain: r
Molecule details ›
Chain: r
Length: 127 amino acids
Theoretical weight: 15.43 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5PZE3 (Residues: 2-128; Coverage: 99%)
Gene name: NDUFB6
Sequence domains: NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit
Mitochondrial complex I, B18 subunit Chain: s
Molecule details ›
Chain: s
Length: 136 amino acids
Theoretical weight: 16.28 KDa
Source organism: Ovis aries
Mitochondrial complex I, B22 subunit Chain: t
Molecule details ›
Chain: t
Length: 178 amino acids
Theoretical weight: 21.65 KDa
Source organism: Ovis aries
Mitochondrial complex I, AGGG subunit Chain: u
Molecule details ›
Chain: u
Length: 72 amino acids
Theoretical weight: 8.5 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: v
Molecule details ›
Chain: v
Length: 158 amino acids
Theoretical weight: 18.82 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q1B0 (Residues: 29-186; Coverage: 85%)
Gene name: NDUFB8
Sequence domains: NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8)
Mitochondrial complex I, ESSS subunit Chain: w
Molecule details ›
Chain: w
Length: 125 amino acids
Theoretical weight: 14.46 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial Chain: x
Molecule details ›
Chain: x
Length: 49 amino acids
Theoretical weight: 5.81 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: Q02376 (Residues: 28-76; Coverage: 65%)
Gene name: NDUFC1
Sequence domains: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 Chain: y
Molecule details ›
Chain: y
Length: 57 amino acids
Theoretical weight: 6.93 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5QG39 (Residues: 2-58; Coverage: 98%)
Gene name: LOC114115567
Sequence domains: MNLL subunit
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Chain: z
Molecule details ›
Chain: z
Length: 70 amino acids
Theoretical weight: 8.21 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: Q02377 (Residues: 1-70; Coverage: 100%)
Gene name: NDUFA1
Sequence domains: NADH-ubiquinone oxidoreductase MWFE subunit
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 Chain: Z
Molecule details ›
Chain: Z
Length: 175 amino acids
Theoretical weight: 20.88 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: W5Q5Y9 (Residues: 9-11, 12-170; Coverage: 95%)
Gene name: NDUFB10
Sequence domains: NADH-ubiquinone oxidoreductase subunit 10
Mitochondrial complex I, PGIV subunit Chain: Y
Molecule details ›
Chain: Y
Length: 171 amino acids
Theoretical weight: 20.01 KDa
Source organism: Ovis aries
Mitochondrial complex I, SGDH subunit Chain: W
Molecule details ›
Chain: W
Length: 143 amino acids
Theoretical weight: 16.71 KDa
Source organism: Ovis aries
Mitochondrial complex I, B14.7 subunit Chain: V
Molecule details ›
Chain: V
Length: 119 amino acids
Theoretical weight: 10.15 KDa
Source organism: Ovis aries

Ligands and Environments

10 bound ligands:
Ligand SF4 6 x SF4
Ligand FMN 1 x FMN
Ligand FES 2 x FES
Ligand 3PE 4 x 3PE
Ligand PC1 4 x PC1
Ligand CDL 4 x CDL
Ligand ZN 1 x ZN
Ligand NDP 1 x NDP
Ligand ZMP 1 x ZMP
Ligand PNS 1 x PNS
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-4093

Quick links

Citations

57 review citations

Clarifying the supercomplex: the higher-order organization of the mitochondrial electron transport chain.
Letts et al. (2017)
56 more

47 mentions without citation

Structure and mechanism of mitochondrial electron transport chain.
Guo et al. (2018)
46 more