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› 5lob

X-ray diffraction
3.3Å resolution

Structure of the Ca2+-bound Rabphilin3A C2B- SNAP25 complex (C2 space group)

Released:
DOI: 10.2210/pdb5lob/pdb
Model geometry
Fit model/data
PDB entry 5lob coloured by chain, front view.
PDB entry 5lob coloured by chain, side view.
PDB entry 5lob coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural characterization of the Rabphilin-3A-SNAP25 interaction.
Ferrer-Orta C, Pérez-Sánchez MD, Coronado-Parra T, Silva C, López-Martínez D, Baltanás-Copado J, Gómez-Fernández JC, Corbalán-García S, Verdaguer N
Proc Natl Acad Sci U S A 114 E5343-E5351 (2017)
PMID: 28634303

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155640 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Rabphilin-3A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 162 amino acids
Theoretical weight: 18.58 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P47709 (Residues: 536-680; Coverage: 21%)
Gene name: Rph3a
Sequence domains: C2 domain
Structure domains: C2 domain
Synaptosomal-associated protein 25 Chains: D, F
Molecule details ›
Chains: D, F
Length: 100 amino acids
Theoretical weight: 11.27 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P60881 (Residues: 7-82; Coverage: 37%)
  • Best match: P60881-2 (Residues: 7-82)
Gene names: Snap, Snap25
Synaptosomal-associated protein 25 Chains: E, G

Ligands and Environments

3 bound ligands:
Ligand CA 6 x CA
Ligand SO4 10 x SO4
Ligand GOL 9 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2
Unit cell:
a: 153.97Å b: 58.754Å c: 125.311Å
α: 90° β: 103.24° γ: 90°
R-values:
R R work R free
0.242 0.24 0.286
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

The Electrophysiology of Presynaptic Congenital Myasthenic Syndromes With and Without Facilitation: From Electrodiagnostic Findings to Molecular Mechanisms.
Nicolau et al. (2019)
16 other articles

1 mention without citation

Structural characterization of the Rabphilin-3A-SNAP25 interaction.
Ferrer-Orta et al. (2017)