› 5lpp

X-ray diffraction
1.99Å resolution

tRNA guanine Transglycosylase (TGT) in co-crystallized complex (space group C2) with 6-amino-4-(2-((3aR,4R,6R,6aR)-6-methoxy-2,2-dimethyltetrahydrofuro[3,4-d][1,3]dioxol-4-yl)ethyl)-2-(methylamino)-1H-imidazo[4,5-g]quinazolin-8(7H)-one

Released:
DOI: 10.2210/pdb5lpp/pdb
PDB entry 5lpp coloured by chain, front view.
PDB entry 5lpp coloured by chain, side view.
PDB entry 5lpp coloured by chain, top view.
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Entry authors: Ehrmann FR, Heine A, Klebe G

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.93 KDa
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Expression system: Escherichia coli
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase
Structure domains: Queuine tRNA-ribosyltransferase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand 72C 1 x 72C
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 85.423Å b: 64.443Å c: 71.223Å
α: 90° β: 93.88° γ: 90°
R-values:
R R work R free
0.182 0.181 0.216
Expression system: Escherichia coli

Quick links