› 5lqb

X-ray diffraction
1.95Å resolution

Complex structure of human IL2 mutant, Proleukin, with Fab fragment of NARA1 antibody

Released:
DOI: 10.2210/pdb5lqb/pdb
PDB entry 5lqb coloured by chain, front view.
PDB entry 5lqb coloured by chain, side view.
PDB entry 5lqb coloured by chain, top view.
Source organisms:
Primary publication:
Improved cancer immunotherapy by a CD25-mimobody conferring selectivity to human interleukin-2.
Arenas-Ramirez N, Zou C, Popp S, Zingg D, Brannetti B, Wirth E, Calzascia T, Kovarik J, Sommer L, Zenke G, Woytschak J, Regnier CH, Katopodis A, Boyman O
Sci Transl Med 8 367ra166 (2016)
PMID: 27903862

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211411 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-2 Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60568 (Residues: 22-153; Coverage: 99%)
Gene name: IL2
Sequence domains: Interleukin 2
Structure domains: Growth Hormone; Chain: A;
anti-hIL2 FAB fragment heavy chain Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
anti-hIL2 FAB fragment light chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 23.93 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 201.757Å b: 36.233Å c: 88.707Å
α: 90° β: 102.93° γ: 90°
R-values:
R R work R free
0.194 0.189 0.291
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Biology and regulation of IL-2: from molecular mechanisms to human therapy.
Spolski et al. (2018)
33 more

4 mentions without citation

A systematic study of traditional Chinese medicine treating hepatitis B virus-related hepatocellular carcinoma based on target-driven reverse network pharmacology.
Yin et al. (2022)
3 more