› 5lrr

X-ray diffraction
2.17Å resolution

The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with glutathione

Released:
DOI: 10.2210/pdb5lrr/pdb
PDB entry 5lrr coloured by chain, front view.
PDB entry 5lrr coloured by chain, side view.
PDB entry 5lrr coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria.
Hall M, Grundström C, Begum A, Lindberg MJ, Sauer UH, Almqvist F, Johansson J, Sauer-Eriksson AE
Proc Natl Acad Sci U S A 113 14733-14738 (2016)
PMID: 27930316

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 237 amino acids
Theoretical weight: 27.33 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 1-237; Coverage: 100%)
Gene names: lmo0200, prfA
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GSH 8 x GSH
Ligand NA 5 x NA
Ligand PI 3 x PI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 116.265Å b: 184.227Å c: 100.457Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.241
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis.
Radoshevich et al. (2018)
13 more

5 mentions without citation

Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria.
Hall et al. (2016)
4 more