› 5lte

X-ray diffraction
1.65Å resolution

Crystal structure of the alpha subunit of heme dependent oxidative N-demethylase (HODM)

Released:
DOI: 10.2210/pdb5lte/pdb
PDB entry 5lte coloured by chain, front view.
PDB entry 5lte coloured by chain, side view.
PDB entry 5lte coloured by chain, top view.
Source organism: Pseudomonas mendocina ymp
Primary publication:
An oxidative N-demethylase reveals PAS transition from ubiquitous sensor to enzyme.
Ortmayer M, Lafite P, Menon BR, Tralau T, Fisher K, Denkhaus L, Scrutton NS, Rigby SE, Munro AW, Hay S, Leys D
Nature 539 593-597 (2016)
PMID: 27851736

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107491 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF3445 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 40.16 KDa
Source organism: Pseudomonas mendocina ymp
Expression system: Escherichia coli
UniProt:
  • Canonical: A4XXY9 (Residues: 1-338; Coverage: 100%)
Gene name: Pmen_3455
Sequence domains: Haem-dependent oxidative N-demethylase, alpha subunit-like

Ligands and Environments

3 bound ligands:
Ligand HEM 1 x HEM
Ligand ETX 1 x ETX
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P43212
Unit cell:
a: 79.908Å b: 79.908Å c: 144.712Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Loop dynamics and the evolution of enzyme activity.
Corbella et al. (2023)
7 other articles

1 mention without citation

Phylogenetic Analysis with Prediction of Cofactor or Ligand Binding for Pseudomonas aeruginosa PAS and Cache Domains.
Hutchin et al. (2021)