› 5luv

X-ray diffraction
2.5Å resolution

Short LOV protein W619_1 in apo-state

Released:
DOI: 10.2210/pdb5luv/pdb
PDB entry 5luv coloured by chain, front view.
PDB entry 5luv coloured by chain, side view.
PDB entry 5luv coloured by chain, top view.
Source organism: Pseudomonas putida W619
Primary publication:
Structure of a LOV protein in apo-state and implications for construction of LOV-based optical tools.
Arinkin V, Granzin J, Röllen K, Krauss U, Jaeger KE, Willbold D, Batra-Safferling R
Sci Rep 7 42971 (2017)
PMID: 28211532

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative PAS/PAC sensor protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 19.12 KDa
Source organism: Pseudomonas putida W619
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1J385 (Residues: 7-152; Coverage: 96%)
Gene name: PputW619_0812
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I41
Unit cell:
a: 109.538Å b: 109.538Å c: 95.209Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Blue-Light Receptors for Optogenetics.
Losi et al. (2018)
1 more

1 mention without citation

Structure of a LOV protein in apo-state and implications for construction of LOV-based optical tools.
Arinkin et al. (2017)