› 5lv4

X-ray diffraction
1.66Å resolution

Crystal structure of mouse PRMT6 in complex with inhibitor LH1236

Released:
DOI: 10.2210/pdb5lv4/pdb
PDB entry 5lv4 coloured by chain, front view.
PDB entry 5lv4 coloured by chain, side view.
PDB entry 5lv4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Hijacking DNA methyltransferase transition state analogues to produce chemical scaffolds for PRMT inhibitors.
Halby L, Marechal N, Pechalrieu D, Cura V, Franchini DM, Faux C, Alby F, Troffer-Charlier N, Kudithipudi S, Jeltsch A, Aouadi W, Decroly E, Guillemot JC, Page P, Ferroud C, Bonnefond L, Guianvarc'h D, Cavarelli J, Arimondo PB
Philos Trans R Soc Lond B Biol Sci 373 (2018)
PMID: 29685976

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein arginine N-methyltransferase 6 Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NZB1 (Residues: 1-378; Coverage: 100%)
Gene names: Hrmt1l6, Prmt6
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 78K 1 x 78K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I41
Unit cell:
a: 79.851Å b: 79.851Å c: 119.738Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.188
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Roles of Host 5-Methylcytosine RNA Methyltransferases during Viral Infections.
Wnuk et al. (2020)
10 other articles

2 mentions without citation

Protein arginine methyltransferases: insights into the enzyme structure and mechanism at the atomic level.
Tewary et al. (2019)
1 more