› 5lwf

X-ray diffraction
2.56Å resolution

Structure of a single domain camelid antibody fragment cAb-G10S in complex with the BlaP beta-lactamase from Bacillus licheniformis

Released:
DOI: 10.2210/pdb5lwf/pdb
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PDB entry 5lwf coloured by chain, front view.
PDB entry 5lwf coloured by chain, side view.
PDB entry 5lwf coloured by chain, top view.
Source organisms:
Entry authors: Vettore N, Kerff F, Pain C, Herman R, Sauvage E, Preumont S, Charlier P, Dumoulin M

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208318 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 30.41 KDa
Source organism: Bacillus licheniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P00808 (Residues: 44-211, 212-307; Coverage: 94%)
Gene names: blaP, penP
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily
Camelid heavy-chain antibody variable fragment cAb-G10S Chains: C, D
Molecule details ›
Chains: C, D
Length: 131 amino acids
Theoretical weight: 14.59 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 47.278Å b: 121.691Å c: 137.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.242
Expression system: Escherichia coli

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