› 5lx0

X-ray diffraction
2.4Å resolution

Cys-Gly dipeptidase GliJ (space group P3221)

Released:
DOI: 10.2210/pdb5lx0/pdb
Model geometry
Fit model/data
PDB entry 5lx0 coloured by chain, front view.
PDB entry 5lx0 coloured by chain, side view.
PDB entry 5lx0 coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Gliotoxin Biosynthesis: Structure, Mechanism, and Metal Promiscuity of Carboxypeptidase GliJ.
Marion A, Groll M, Scharf DH, Scherlach K, Glaser M, Sievers H, Schuster M, Hertweck C, Brakhage AA, Antes I, Huber EM
ACS Chem Biol 12 1874-1882 (2017)
PMID: 28525266

Function and Biology Details

Reaction catalysed: 
Hydrolysis of dipeptides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175833 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dipeptidase gliJ Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 44.88 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4WMJ8 (Residues: 1-387; Coverage: 100%)
Gene names: AFUA_6G09650, gliJ
Sequence domains: Membrane dipeptidase (Peptidase family M19)
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 100.01Å b: 100.01Å c: 107.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.195
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action.
Lyagin et al. (2019)
3 more