› 5lxf

X-ray diffraction
2Å resolution

Crystal structure of the human Macrophage Colony Stimulating Factor M- CSF_C31S variant

Released:
DOI: 10.2210/pdb5lxf/pdb
PDB entry 5lxf coloured by chain, front view.
PDB entry 5lxf coloured by chain, side view.
PDB entry 5lxf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering a monomeric variant of macrophage colony-stimulating factor (M-CSF) that antagonizes the c-FMS receptor.
Zur Y, Rosenfeld L, Bakhman A, Ilic S, Hayun H, Shahar A, Akabayov B, Kosloff M, Levaot N, Papo N
Biochem J 474 2601-2617 (2017)
PMID: 28655719

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage colony-stimulating factor 1 43 kDa subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 21.35 KDa
Source organism: Homo sapiens
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: P09603 (Residues: 33-181; Coverage: 29%)
Gene name: CSF1
Sequence domains: Macrophage colony stimulating factor-1 (CSF-1)
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 33.271Å b: 65.471Å c: 158.632Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.253
Expression system: Komagataella phaffii GS115

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Citations

7 citation in other articles

Interplay between negative and positive design elements in Gα helical domains of G proteins determines interaction specificity toward RGS2.
Kasom et al. (2018)
6 more