5lxu

X-ray diffraction
2.14Å resolution

Structure of the DNA-binding domain of LUX ARRHYTHMO

Released:
Source organism: Arabidopsis thaliana
Entry authors: Zubieta C, Nanao MH

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-119000 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor LUX Chain: A
Molecule details ›
Chain: A
Length: 57 amino acids
Theoretical weight: 6.81 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SNB4 (Residues: 144-200; Coverage: 18%)
Gene names: At3g46640, F12A12.160, LUX, PCL1
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
DNA (5'-D(*AP*TP*GP*CP*GP*TP*AP*TP*CP*TP*TP*AP*GP*AP*TP*AP*CP*GP*CP*A)-3') Chain: U
Molecule details ›
Chain: U
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
DNA (5'-D(*AP*TP*GP*CP*GP*TP*AP*TP*CP*TP*TP*AP*GP*AP*TP*AP*CP*GP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P2
Unit cell:
a: 42.16Å b: 32.83Å c: 53.76Å
α: 90° β: 98.61° γ: 90°
R-values:
R R work R free
0.197 0.195 0.232
Expression systems:
  • Escherichia coli
  • Not provided