› 5lye

X-ray diffraction
1.9Å resolution

Re-refined structure of the bacteriophage T4 short tail fibre PDB entry 1H6W containing 71 additionally identified residues

Released:
DOI: 10.2210/pdb5lye/pdb
PDB entry 5lye coloured by chain, front view.
PDB entry 5lye coloured by chain, side view.
PDB entry 5lye coloured by chain, top view.
Source organism: Escherichia virus T2
Primary publication:
Contractile injection systems of bacteriophages and related systems.
Taylor NMI, van Raaij MJ, Leiman PG
Mol Microbiol 108 6-15 (2018)
PMID: 29405518

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short tail fiber protein Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 33.67 KDa
Source organism: Escherichia virus T2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q38160 (Residues: 85-398; Coverage: 60%)
Gene name: 12
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P321
Unit cell:
a: 51.257Å b: 51.257Å c: 249.491Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.215
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Evolutionary entanglement of mobile genetic elements and host defence systems: guns for hire.
Koonin et al. (2020)
10 more