› 5m09

X-ray diffraction
2.98Å resolution

Crystal structure of Mycobacterium tuberculosis PknI kinase domain, C20A_R136N double mutant

Released:
DOI: 10.2210/pdb5m09/pdb
Model geometry
Fit model/data
PDB entry 5m09 coloured by chain, front view.
PDB entry 5m09 coloured by chain, side view.
PDB entry 5m09 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The crystal structure of PknI from Mycobacterium tuberculosis shows an inactive, pseudokinase-like conformation.
Lisa MN, Wagner T, Alexandre M, Barilone N, Raynal B, Alzari PM, Bellinzoni M
FEBS J 284 602-614 (2017)
PMID: 28054744

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknI Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 29.6 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WI69 (Residues: 1-256; Coverage: 44%)
Gene names: MTCY338.02c, Rv2914c, pknI
Sequence domains: Protein kinase domain
Structure domains: Phosphorylase Kinase; domain 1

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P4322
Unit cell:
a: 110.87Å b: 110.87Å c: 181.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Mycobacterium tuberculosis serine/threonine protein kinases: structural information for the design of their specific ATP-competitive inhibitors.
Caballero et al. (2018)
1 more

1 mention without citation

The crystal structure of PknI from Mycobacterium tuberculosis shows an inactive, pseudokinase-like conformation.
Lisa et al. (2017)