5m1s

Electron Microscopy
6.7Å resolution

Cryo-EM structure of the E. coli replicative DNA polymerase-clamp-exonuclase-theta complex bound to DNA in the editing mode

Released:
Source organism: Escherichia coli K-12
Primary publication:
Self-correcting mismatches during high-fidelity DNA replication.
Nat Struct Mol Biol 24 140-143 (2017)
PMID: 28067916
Related structures: EMD-4141

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-119003 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
DNA polymerase III subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 927 amino acids
Theoretical weight: 103.55 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10443 (Residues: 1-927; Coverage: 80%)
Gene names: JW0179, b0184, dnaE, polC
Sequence domains:
Beta sliding clamp Chains: B, C
Molecule details ›
Chains: B, C
Length: 366 amino acids
Theoretical weight: 40.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A988 (Residues: 1-366; Coverage: 100%)
Gene names: JW3678, b3701, dnaN
Sequence domains:
DNA polymerase III subunit epsilon Chain: D
Molecule details ›
Chain: D
Length: 243 amino acids
Theoretical weight: 27.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03007 (Residues: 1-243; Coverage: 100%)
Gene names: JW0205, b0215, dnaQ, mutD
Sequence domains: Exonuclease
DNA polymerase III subunit theta Chain: F
Molecule details ›
Chain: F
Length: 56 amino acids
Theoretical weight: 6.5 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABS8 (Residues: 10-65; Coverage: 74%)
Gene names: JW1831, b1842, holE
Sequence domains: DNA polymerase III, theta subunit
DNA Primer Strand Chain: P
Molecule details ›
Chain: P
Length: 17 nucleotides
Theoretical weight: 5.28 KDa
DNA Template Strand Chain: T
Molecule details ›
Chain: T
Length: 22 nucleotides
Theoretical weight: 6.72 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.7Å
Relevant EMDB volumes: EMD-4141
Expression system: Escherichia coli BL21(DE3)