› 5m2s

X-ray diffraction
1.7Å resolution

R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin

Released:
DOI: 10.2210/pdb5m2s/pdb
PDB entry 5m2s coloured by chain, front view.
PDB entry 5m2s coloured by chain, side view.
PDB entry 5m2s coloured by chain, top view.
Source organisms:
Primary publication:
Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes.
Bule P, Alves VD, Israeli-Ruimy V, Carvalho AL, Ferreira LMA, Smith SP, Gilbert HJ, Najmudin S, Bayer EA, Fontes CMGA
Sci Rep 7 759 (2017)
PMID: 28389644

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106041 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative cellulosomal scaffoldin protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 15.93 KDa
Source organism: Ruminococcus flavefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0AEF3 (Residues: 465-610; Coverage: 21%)
Gene name: scaA
Structure domains: Immunoglobulin-like
Doc8: Type I dockerin repeat domain from family 9 glycoside hydrolase WP_009982745[Ruminococcus flavefaciens] Chain: B
Molecule details ›
Chain: B
Length: 103 amino acids
Theoretical weight: 10.97 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 45.61Å b: 64.49Å c: 47.67Å
α: 90° β: 116.72° γ: 90°
R-values:
R R work R free
0.135 0.134 0.157
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Surfaceome and Proteosurfaceome in Parietal Monoderm Bacteria: Focus on Protein Cell-Surface Display.
Desvaux et al. (2018)
4 more

2 mentions without citation

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes.
Bule et al. (2017)
1 more