› 5m3c

X-ray diffraction
2.8Å resolution

Structure of the hybrid domain (GGDEF-EAL) of PA0575 from Pseudomonas aeruginosa PAO1 at 2.8 Ang. with GTP and Ca2+ bound to the active site of the GGDEF domain

Released:
DOI: 10.2210/pdb5m3c/pdb
PDB entry 5m3c coloured by chain, front view.
PDB entry 5m3c coloured by chain, side view.
PDB entry 5m3c coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa.
Mantoni F, Paiardini A, Brunotti P, D'Angelo C, Cervoni L, Paone A, Cappellacci L, Petrelli R, Ricciutelli M, Leoni L, Rampioni G, Arcovito A, Rinaldo S, Cutruzzolà F, Giardina G
FEBS J 285 3815-3834 (2018)
PMID: 30106221

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional diguanylate cyclase/phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 49.08 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I5W1 (Residues: 809-1245; Coverage: 36%)
Gene name: PA0575
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 5 x CA
Ligand GTP 2 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Spacegroup: P21212
Unit cell:
a: 122.307Å b: 133.931Å c: 69.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.244 0.277
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Biofilm Maintenance as an Active Process: Evidence that Biofilms Work Hard to Stay Put.
Katharios-Lanwermeyer et al. (2022)
1 more

2 mentions without citation

Studying GGDEF Domain in the Act: Minimize Conformational Frustration to Prevent Artefacts.
Mantoni et al. (2021)
1 more