› 5m46

X-ray diffraction
1.62Å resolution

Alpha-amino epsilon-caprolactam racemase (ACLR) from Rhizobacterium freirei

Released:
DOI: 10.2210/pdb5m46/pdb
PDB entry 5m46 coloured by chain, front view.
PDB entry 5m46 coloured by chain, side view.
PDB entry 5m46 coloured by chain, top view.
Source organism: Rhizobium freirei PRF 81
Primary publication:
Snapshots of the Catalytic Cycle of the Industrial Enzyme alpha-Amino-epsilon-Caprolactam Racemase (ACLR) Observed Using X-ray Crystallography
Frese A, Sutton PW, Turkenburg JP, Grogan G
ACS Catalysis 7 1045-1048 (2017)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class-III Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 46.05 KDa
Source organism: Rhizobium freirei PRF 81
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: N6UXY4 (Residues: 1-436; Coverage: 100%)
Gene name: RHSP_08934
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PLP 1 x PLP
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 87.613Å b: 76.527Å c: 58.57Å
α: 90° β: 113.12° γ: 90°
R-values:
R R work R free
0.149 0.147 0.18
Expression system: Escherichia coli BL21(DE3)

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