› 5m58

X-ray diffraction
2.05Å resolution

Crystal structure of CouO, a C-methyltransferase from Streptomyces rishiriensis

Released:
DOI: 10.2210/pdb5m58/pdb
PDB entry 5m58 coloured by chain, front view.
PDB entry 5m58 coloured by chain, side view.
PDB entry 5m58 coloured by chain, top view.
Source organism: Streptomyces rishiriensis
Primary publication:
Crystal Structure and Catalytic Mechanism of CouO, a Versatile C-Methyltransferase from Streptomyces rishiriensis.
Pavkov-Keller T, Steiner K, Faber M, Tengg M, Schwab H, Gruber-Khadjawi M, Gruber K
PLoS One 12 e0171056 (2017)
PMID: 28152088

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-methyltransferase CouO Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 25.68 KDa
Source organism: Streptomyces rishiriensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F8T9 (Residues: 1-230; Coverage: 100%)
Gene name: couO
Sequence domains: Methyltransferase domain

Ligands and Environments

1 bound ligand:
Ligand SAH 2 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P21
Unit cell:
a: 33.07Å b: 82.95Å c: 76.88Å
α: 90° β: 96.91° γ: 90°
R-values:
R R work R free
0.207 0.203 0.264
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Diversity of the reaction mechanisms of SAM-dependent enzymes.
Sun et al. (2021)
2 more

2 mentions without citation

Crystal Structure and Catalytic Mechanism of CouO, a Versatile C-Methyltransferase from Streptomyces rishiriensis.
Pavkov-Keller et al. (2017)
1 more