› 5m61

X-ray diffraction
1.84Å resolution

Clathrin heavy chain N-terminal domain bound to an extended amphiphysin clathrin-box motif

Released:
DOI: 10.2210/pdb5m61/pdb
PDB entry 5m61 coloured by chain, front view.
PDB entry 5m61 coloured by chain, side view.
PDB entry 5m61 coloured by chain, top view.
Source organisms:
Primary publication:
Cellular and viral peptides bind multiple sites on the N-terminal domain of clathrin.
Muenzner J, Traub LM, Kelly BT, Graham SC
Traffic 18 44-57 (2017)
PMID: 27813245

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155946 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Clathrin heavy chain 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 40.54 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49951 (Residues: 1-363; Coverage: 22%)
Gene name: CLTC
Sequence domains:
Structure domains: Clathrin heavy-chain terminal domain
Amphiphysin Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 12 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P49418 (Residues: 349-360; Coverage: 2%)
Gene names: AMPH, AMPH1

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 138.12Å b: 131.15Å c: 77.83Å
α: 90° β: 115.35° γ: 90°
R-values:
R R work R free
0.162 0.161 0.182
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Behaviour of intrinsically disordered proteins in protein-protein complexes with an emphasis on fuzziness.
Olsen et al. (2017)
5 more