› 5m6c

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF T71N MUTANT OF HUMAN HIPPOCALCIN

Released:
DOI: 10.2210/pdb5m6c/pdb
PDB entry 5m6c coloured by chain, front view.
PDB entry 5m6c coloured by chain, side view.
PDB entry 5m6c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Biophysical and functional characterization of hippocalcin mutants responsible for human dystonia.
Helassa N, Antonyuk SV, Lian LY, Haynes LP, Burgoyne RD
Hum Mol Genet 26 2426-2435 (2017)
PMID: 28398555

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160792 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neuron-specific calcium-binding protein hippocalcin Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 22.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84074 (Residues: 1-193; Coverage: 100%)
Gene names: BDR2, HPCA
Sequence domains:
Neuron-specific calcium-binding protein hippocalcin Chain: E
Molecule details ›
Chain: E
Length: 193 amino acids
Theoretical weight: 22.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P84074 (Residues: 1-193; Coverage: 100%)
Gene names: BDR2, HPCA
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P61
Unit cell:
a: 51.16Å b: 51.16Å c: 284.061Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.219 0.283
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli BL21

Quick links

Citations

5 review citations

Dystonia.
Balint et al. (2018)
4 more

3 mentions without citation

Calcium Sensors in Neuronal Function and Dysfunction.
Burgoyne et al. (2019)
2 more