› 5m7o

X-ray diffraction
2.2Å resolution

Crystal structure of NtrX from Brucella abortus processed with the CrystalDirect automated mounting and cryo-cooling technology

Released:
DOI: 10.2210/pdb5m7o/pdb
PDB entry 5m7o coloured by chain, front view.
PDB entry 5m7o coloured by chain, side view.
PDB entry 5m7o coloured by chain, top view.
Source organism: Brucella abortus str. 2308 A
Primary publication:
Three-Dimensional Structure of Full-Length NtrX, an Unusual Member of the NtrC Family of Response Regulators.
Fernández I, Cornaciu I, Carrica MD, Uchikawa E, Hoffmann G, Sieira R, Márquez JA, Goldbaum FA
J Mol Biol 429 1192-1212 (2017)
PMID: 28088479

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen assimilation regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 454 amino acids
Theoretical weight: 50.28 KDa
Source organism: Brucella abortus str. 2308 A
Expression system: Escherichia coli
UniProt:
  • Canonical: C4IRH0 (Residues: 1-453; Coverage: 100%)
Gene name: BAAA_2000042
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 124.778Å b: 191.228Å c: 111.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.269
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Role of the Extremolytes Ectoine and Hydroxyectoine as Stress Protectants and Nutrients: Genetics, Phylogenomics, Biochemistry, and Structural Analysis.
Czech et al. (2018)
13 other articles

1 mention without citation

Assessing the Influence of Mutation on GTPase Transition States by Using X-ray Crystallography, 19 F NMR, and DFT Approaches.
Jin et al. (2017)