› 5m8e

X-ray diffraction
2Å resolution

Crystal structure of a GH43 arabonofuranosidase from Weissella sp. strain 142

Released:
DOI: 10.2210/pdb5m8e/pdb
PDB entry 5m8e coloured by chain, front view.
PDB entry 5m8e coloured by chain, side view.
PDB entry 5m8e coloured by chain, top view.
Source organism: Weissella cibaria
Primary publication:
Three-dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis.
Linares-Pastén JA, Falck P, Albasri K, Kjellström S, Adlercreutz P, Logan DT, Karlsson EN
FEBS J 284 2019-2036 (2017)
PMID: 28485897

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101102 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extracellular exo-alpha-(1->5)-L-arabinofuranosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 344 amino acids
Theoretical weight: 39.82 KDa
Source organism: Weissella cibaria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0D1M7L2 (Residues: 1-324; Coverage: 100%)
Gene names: B6254_2263, B9D04_01605, QX99_00224
Sequence domains: Glycosyl hydrolases family 43
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand IMD 2 x IMD
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21
Unit cell:
a: 59.88Å b: 71.93Å c: 79.51Å
α: 90° β: 101.93° γ: 90°
R-values:
R R work R free
0.175 0.172 0.216
Expression system: Escherichia coli BL21(DE3)

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Citations

7 citation in other articles

Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres.
Månberger et al. (2020)
6 more

1 mention without citation

Three-dimensional structures and functional studies of two GH43 arabinofuranosidases from Weissella sp. strain 142 and Lactobacillus brevis.
Linares-Pastén et al. (2017)