› 5m94

X-ray diffraction
3.1Å resolution

Crystal structure of Staphylococcus capitis divalent metal ion transporter (DMT) in complex with nanobody

Released:
DOI: 10.2210/pdb5m94/pdb
PDB entry 5m94 coloured by chain, front view.
PDB entry 5m94 coloured by chain, side view.
PDB entry 5m94 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.
Ehrnstorfer IA, Geertsma ER, Pardon E, Steyaert J, Dutzler R
Nat Struct Mol Biol 21 990-6 (2014)
PMID: 25326704

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-225807 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Divalent metal cation transporter MntH Chains: A, C
Molecule details ›
Chains: A, C
Length: 415 amino acids
Theoretical weight: 45.51 KDa
Source organism: Staphylococcus capitis
Expression system: Escherichia coli
CAMELID ANTIBODY FRAGMENT, NANOBODY Chains: B, D
Molecule details ›
Chains: B, D
Length: 123 amino acids
Theoretical weight: 13.44 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 114.35Å b: 114.35Å c: 257.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.237 0.273
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Targeting iron metabolism in drug discovery and delivery.
Crielaard et al. (2017)
16 more

6 mentions without citation

Molecular Mechanism of Nramp-Family Transition Metal Transport.
Bozzi et al. (2021)
5 more