› 5m95

X-ray diffraction
3.4Å resolution

STAPHYLOCOCCUS CAPITIS DIVALENT METAL ION TRANSPORTER (DMT) IN COMPLEX WITH MANGANESE

Released:
DOI: 10.2210/pdb5m95/pdb
PDB entry 5m95 coloured by chain, front view.
PDB entry 5m95 coloured by chain, side view.
PDB entry 5m95 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.
Ehrnstorfer IA, Geertsma ER, Pardon E, Steyaert J, Dutzler R
Nat Struct Mol Biol 21 990-6 (2014)
PMID: 25326704

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-211717 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Divalent metal cation transporter MntH Chains: A, C
Molecule details ›
Chains: A, C
Length: 415 amino acids
Theoretical weight: 45.52 KDa
Source organism: Staphylococcus capitis
Expression system: Escherichia coli
CAMELID ANTIBODY FRAGMENT, NANOBODY Chains: B, D
Molecule details ›
Chains: B, D
Length: 124 amino acids
Theoretical weight: 13.53 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 114.17Å b: 114.17Å c: 257.53Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.252 0.292
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Camelid nanobodies: killing two birds with one stone.
Desmyter et al. (2015)
16 more

6 mentions without citation

Molecular Mechanism of Nramp-Family Transition Metal Transport.
Bozzi et al. (2021)
5 more