› 5mac

X-ray diffraction
2.6Å resolution

Crystal structure of decameric Methanococcoides burtonii Rubisco complexed with 2-carboxyarabinitol bisphosphate

Released:
DOI: 10.2210/pdb5mac/pdb
PDB entry 5mac coloured by chain, front view.
PDB entry 5mac coloured by chain, side view.
PDB entry 5mac coloured by chain, top view.
Source organism: Methanococcoides burtonii
Primary publication:
A unique structural domain in Methanococcoides burtonii ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) acts as a small subunit mimic.
Gunn LH, Valegård K, Andersson I
J Biol Chem 292 6838-6850 (2017)
PMID: 28154188

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribulose-1,5-bisphosphate carboxylase-oxygenase type III Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 474 amino acids
Theoretical weight: 52.96 KDa
Source organism: Methanococcoides burtonii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q12TQ0 (Residues: 1-474; Coverage: 100%)
Gene name: Mbur_2322
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CAP 5 x CAP
Ligand MG 10 x MG
Ligand CL 5 x CL
1 modified residue:
Ligand KCX 5 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P321
Unit cell:
a: 273.76Å b: 273.76Å c: 96.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

A short history of RubisCO: the rise and fall (?) of Nature's predominant CO2 fixing enzyme.
Erb et al. (2018)
3 more

3 mentions without citation

A unique structural domain in Methanococcoides burtonii ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) acts as a small subunit mimic.
Gunn et al. (2017)
2 more