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› 5mal

X-ray diffraction
1.71Å resolution

Crystal structure of extracelular lipase from Streptomyces rimosus at 1.7A resolution

Released:
DOI: 10.2210/pdb5mal/pdb
Model geometry
Fit model/data
PDB entry 5mal coloured by chain, front view.
PDB entry 5mal coloured by chain, side view.
PDB entry 5mal coloured by chain, top view.
Source organism: Streptomyces rimosus
Primary publication:
Catalytic Dyad in the SGNH Hydrolase Superfamily: In-depth Insight into Structural Parameters Tuning the Catalytic Process of Extracellular Lipase from Streptomyces rimosus.
Leščić Ašler I, Štefanić Z, Maršavelski A, Vianello R, Kojić-Prodić B
ACS Chem Biol 12 1928-1936 (2017)
PMID: 28558229

Function and Biology Details

Reactions catalysed: 
a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+).
hexadecanoyl-CoA + H2O = hexadecanoate + CoA + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 24.19 KDa
Source organism: Streptomyces rimosus
UniProt:
  • Canonical: Q93MW7 (Residues: 35-268; Coverage: 100%)
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 38.09Å b: 78.69Å c: 56.56Å
α: 90° β: 104.49° γ: 90°
R-values:
R R work R free
0.168 0.165 0.214

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Citations

3 review citations

Microbial esterases and ester prodrugs: An unlikely marriage for combating antibiotic resistance.
Larsen et al. (2019)
2 more

5 mentions without citation

Catalytic Dyad in the SGNH Hydrolase Superfamily: In-depth Insight into Structural Parameters Tuning the Catalytic Process of Extracellular Lipase from Streptomyces rimosus.
Leščić Ašler et al. (2017)
4 more