› 5mbc

X-ray diffraction
1.8Å resolution

Structure of a bacterial light-regulated adenylyl cylcase

Released:
DOI: 10.2210/pdb5mbc/pdb
PDB entry 5mbc coloured by chain, front view.
PDB entry 5mbc coloured by chain, side view.
PDB entry 5mbc coloured by chain, top view.
Source organism: Beggiatoa sp. PS
Primary publication:
Photoactivation Mechanism of a Bacterial Light-Regulated Adenylyl Cyclase.
Lindner R, Hartmann E, Tarnawski M, Winkler A, Frey D, Reinstein J, Meinhart A, Schlichting I
J Mol Biol 429 1336-1351 (2017)
PMID: 28336405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237273 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta subunit of photoactivated adenylyl cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 40.13 KDa
Source organism: Beggiatoa sp. PS
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 47.95Å b: 56.67Å c: 78.09Å
α: 104.2° β: 104.01° γ: 102.54°
R-values:
R R work R free
0.191 0.19 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Blue-Light Receptors for Optogenetics.
Losi et al. (2018)
7 more