› 5mbk

X-ray diffraction
2.4Å resolution

Structure of a bacterial light-regulated adenylyl cylcase

Released:
DOI: 10.2210/pdb5mbk/pdb
PDB entry 5mbk coloured by chain, front view.
PDB entry 5mbk coloured by chain, side view.
PDB entry 5mbk coloured by chain, top view.
Source organism: Beggiatoa sp. PS
Primary publication:
Photoactivation Mechanism of a Bacterial Light-Regulated Adenylyl Cyclase.
Lindner R, Hartmann E, Tarnawski M, Winkler A, Frey D, Reinstein J, Meinhart A, Schlichting I
J Mol Biol 429 1336-1351 (2017)
PMID: 28336405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta subunit of photoactivated adenylyl cyclase Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.11 KDa
Source organism: Beggiatoa sp. PS
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 69.6Å b: 143.36Å c: 57.48Å
α: 90° β: 135.91° γ: 90°
R-values:
R R work R free
0.212 0.209 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Blue-Light Receptors for Optogenetics.
Losi et al. (2018)
7 more

2 mentions without citation

Selective cysteines oxidation in soluble guanylyl cyclase catalytic domain is involved in NO activation.
Alapa et al. (2021)
1 more