› 5mdo

X-ray diffraction
1.95Å resolution

Crystal structure of in vitro folded Chitoporin VhChip from Vibrio harveyi (crystal form I)

Released:
DOI: 10.2210/pdb5mdo/pdb
PDB entry 5mdo coloured by chain, front view.
PDB entry 5mdo coloured by chain, side view.
PDB entry 5mdo coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Structural basis for chitin acquisition by marine Vibrio species.
Aunkham A, Zahn M, Kesireddy A, Pothula KR, Schulte A, Baslé A, Kleinekathöfer U, Suginta W, van den Berg B
Nat Commun 9 220 (2018)
PMID: 29335469

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 352 amino acids
Theoretical weight: 39.19 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: L0RVU0 (Residues: 26-375; Coverage: 100%)
Gene name: chiP
Sequence domains: Gram-negative porin

Ligands and Environments

2 bound ligands:
Ligand NA 18 x NA
Ligand C8E 17 x C8E
1 modified residue:
Ligand MSE 60 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 146.884Å b: 123.379Å c: 147.026Å
α: 90° β: 117.89° γ: 90°
R-values:
R R work R free
0.167 0.167 0.187
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural features underlying recognition and translocation of extracellular polysaccharides.
Zimmer J. (2019)
1 more

6 mentions without citation

Structural basis for chitin acquisition by marine Vibrio species.
Aunkham et al. (2018)
5 more